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KMID : 1024520140230030483
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Journal of the Environmental Sciences
2014 Volume.23 No. 3 p.483 ~ p.492
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Genotoxicity (DNA damage) on Blood Cells of Parrot Fish (Oplegnathus fasciatus) Exposed to Acidified Seawater Making of CO2
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Choi Tae Seob
Lee Ji-Hye
Sung Chan-Gyoung
Lee Jung-Suk
Park Young-Gyu
Kang Seong-Gil
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Abstract
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DNA damage such as genotoxicity was identified with comet assay, which blood cell of a marine parrot fish (Oplegnathus
fasciatus) was exposed to an acidified seawater, lowered pH gradient making of CO2 gas. The gradient of pH were 8.22, 8.03,7.81, 7.55 with control as HBSS solution with pH 7.4. DNA tail moment of fish blood cell was 0.548 ± 0.071 exposed
seawater of pH 8.22 condition, on the other hand, DNA tail moment 1.601 ± 0.197 exposed acidified seawater of pH 7.55
lowest condition. The approximate difference with level of DNA damage was 2.9 times between highest and lowest of pH.
DNA damage with decreasing pH was significantly increased with DNA tail moment on blood cell of marine fish (ANOVA, p
< 0.001). Ocean acidification, especially inducing the leakage of sequestered CO2 in geological structure is a consequence from the burning of fossil fuels, and long term effects on marine habitats and organisms are not fully investigated. The physiological effects on adult fish species are even less known. This result shown that the potential of dissolved CO2 in seawater was revealed to induce the toxic effect on genotoxicity such as DNA breakage.
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KEYWORD
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Fish, Carbon dioxide, Seawater pH, Genotoxicity, DNA damage, Comet assay
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